Coding

Part:BBa_K5048058:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-23)


LAP-6x His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1429
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2472
    Illegal BglII site found at 2570
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1429
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1429
    Illegal NgoMIV site found at 678
    Illegal NgoMIV site found at 1944
    Illegal AgeI site found at 310
    Illegal AgeI site found at 1966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2292


Design Notes

LAP(Listeria Adhesion Protein) from a kind of non-pathogenic Listeria that can interact with HSP60 and help our engineered bacteria adhere to the small intestine(Fig 1).


Fig 1 The expected effect for the Adhesion module, which adheres to the engineering bacteria and small intestine

Source

Listeria innocua strain CFSAN044836 chromosome, complete genomeSequence, ID: CP045743.1,Range 1: 55424 to 58024


References